Basebraid: AB1 Traces

Read real .ab1 and SCF traces on device: trim by quality, flag heterozygotes, align to a reference, build forward/reverse consensus. One purchase.

Basebraid reads your Sanger sequencing traces on iPhone and iPad: real ABIF (.ab1, .abi) and SCF files, decoded on device, with the tools labs usually pay a yearly licence for.

WHAT'S INCLUDED

• Real file parsing: the ABIF tag directory (analysed DATA channels in FWO_ order, PBAS base calls, PLOC peak positions, PCON qualities, run details) and SCF versions 2 and 3
• Four-channel chromatogram with base calls, per-base quality bars, horizontal zoom and trace height
• Quality trimming by the modified Mott algorithm (error-probability limit) or a sliding window; trimmed ends are shaded and left out of exports
• Mixed-peak / heterozygote flagging: a base is flagged when a second channel reaches your chosen share of the called peak, with the IUPAC code suggested
• Base editing with A, C, G, T, N or ambiguity codes; edits are stored beside the file, never written into it, and can be reverted
• Reverse-complement view of the whole trace
• Alignment to a reference you paste (plain or FASTA): local alignment in both orientations, identity, covered span, and every substitution, insertion, deletion and ambiguous call in reference coordinates
• Forward/reverse consensus: the reverse read is reverse-complemented, the overlap aligned, and disagreements resolved by quality or written as IUPAC codes
• Export FASTA, FASTQ (Phred+33), a per-base quality CSV, an alignment report and a PDF report through the share sheet
• Library of imported traces with quality figures, plus "Open in Basebraid" from Files and other apps
• Run log of every import, trim, edit, alignment, consensus and export, exportable as text
• An app-generated sample pair and reference so you can try everything before opening your own data

ONE PURCHASE, PRIVATE

• No subscription or in-app purchase
• No account, advertising, analytics or tracking
• Works offline; traces leave the device only when you export or share them

Research and education use. Confirm calls, edits and consensus against the original traces and your laboratory's validated workflow.